6-p-Toluidino-2-naphthalenesulfonic acid

Product ID: M0704

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Fluorescent probe for conformation and quantitation studies of proteins. It is practically nonfluorescent in water, becoming fluorescent when bound to membranes (q.y. ~0.27) or proteins (q.y. ~0.72).

Protein folding, conformational changes or other processes that modify the exposure of the probe to water can be monitored using this environmentally sensitive dye. TNS is a fluorescent probe useful for measuring conformational changes and quantitative studies of protein hydrophobicity. It is practically nonfluorescent in water, becoming fluorescent when bound to membranes (q.y. ~0.27) or hydrophobic domains of proteins (q.y. ~0.72). The fluorescence is also enhanced by cyclodextrins, permitting a sensitive method for separating and analyzing cyclodextrins with capillary electrophoresis.

Technical Data
SKU M0704
CAS Number 7724-15-4
Unit Size 500mg
Alternative Names TNS
Absorption 318nm
Emission Wavelength 443
Extinction 26
Detection Method Fluorescence
Molecular Formula C₁₇H₁₅NO₃S
Molecular Weight 313.37
Soluble In DMSO, DMF, H2O (pH six+)
Storage Conditions 2-8C, Desiccated, Protect From Light
Notes Ex=318nm

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References and Citations


  • Aziz A, Santhoshkumar P, Sharma KK, Abraham EC. (2007)"Cleavage of the C-terminal serine of human alphaA-crystallin produces alphaA1-172 with increased chaperone activity and oligomeric size." Biochemistry. 46(9):2510-9.
  • Lampe JN, Atkins WM. (2006) "Time-resolved fluorescence studies of heterotropic ligand binding to cytochrome P450 3A4." Biochemistry. 45(40):12204-15.
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  • "Fluorescent Probes for Conformational States of Proteins. I. Mechanism of Fluorescence of 2-p-Toluidinylnaphthalene-6-sulfonate, a Hydrophobic Probe" McClure, W.O., Edelman, G.M. Biochemistry 5(6) (1966) 1908-1919.
  • "Determination of depolymerization kinetics of amylose, amylopectin, and soluble starch by aspergillus oryzae alpha-amylase using a fluorimetric 2-p-toluidinylnaphthalene-6-sulfonate/flow-injection analysis system." Batlle N, Carbonell JV, Sendra JM. Biotechnol Bioeng 70 (2000) 544-552.
  • "Kinetics and Equilibrium Binding of the Dyes TNS and RH 421 to Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase (RUBISCO)." Frank J, et al. J. Chem. Soc. Faraday Trans. 93 (1997) 2379.
  • "Use of 6-p-Toluidino-2-Naphthalenesulfonic Acid to Quantitate Lipids After Thin-Layer Chromatography." Jones M, Keenan RW, Horowitz P. J. Chromatogr. 237 (1982) 522.
  • "pH-Induced conformational changes in Clostridium difficile toxin B." QaDan M, Spyres LM, Ballard JD. Infect. Immun. 68 (2000) 2470-2474.
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