Product M0951

pCMVb Mammalian lacZ Vector

Overview:

This high copy number eukaryotic vector, pCMVb expresses the full-length b-galactosidase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. This vector is very useful for transfection of mammalian cells in culture and for use in other species. The b-galactosidase enzyme expression is enhanced by elements including: SD/SA-RNA splice donor and acceptor sequence, and SV40 late polyadenylylation signal. pCMVb expression vector also contains b-lactamase gene, which acts as a selection marker (100mg/mL ampicillin resistance) in E. coli host. pCMVb vector has been tested to generate up to 2530u/mg cell extract (MacGregor, and Caskey). In addition, the b-galactosidase gene can be excised using the NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our pSV40b vector system, Product M0952.

Full Length Sequence Data

Restriction Sites

Price: 20mg $99.75 (USD)

References:

1. MacGregor, G.R. & Caskey, C.T., 1989. “Construction of Plasmids that Express E. Coli Beta-galactosidase in Mammalian Cells.” Nucleic Acids Res. 17: 2365.
2. Norton, P.A.& Coffin, J.M., 1984. “Bacterial b-galactosidase as a Marker of Rous Sarcoma Virus Gene Expression and Replication.” Mol. Cell Biol. 5: 281.
3. Nolan, G.P., et al., 1988. “Fluorescence-activated Cell Analysis and Sorting of Viable Mammalian Cells Based on b-D-galactosidase Activity After Transduction of Escherichia coli lacZ.” Proc. Natl. Acad. Sci (USA). 85: 2603.