pCMVbeta, 7164 bp. (CIRCULAR)
Restriction analysis 2/4/2004
pDRAW32 revision 1.1.61
Rebase containing 242 enzymes.
43 enzymes match enzyme selection criteria.
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AfeI
(AGC'GCT)
[Eco47III,Aor51HI,FunI]
Cuts 1 time.
Cuts at position 2791.
AhdI
(GACnn_n'nnGTC)
[Eam1105I,AspEI,EclHKI,NruGI]
Cuts 1 time.
Cuts at position 5782.
BaeI
(ACnnnnGTAyCnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 1180.
BaeI
(GrTACnnnnGTnnnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 1213.
BbeI
(G_GCGC'C)
[NarI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 7008.
BclI
(T'GATC_A)
[BsiQI,FbaI,Ksp22I]
[dam methylated]
Cuts 1 time.
Cuts at position [2302].
BlpI
(GC'TnA_GC) [Bpu1102I,Bsp1720I,CelII]
Cuts 1 time.
Cuts at position 3969.
Bpu10I
(CC'TnA_GC)
Cuts 1 time.
Cuts at position 1461.
BsaI
(GGTCTCn'nnnn_)
[Eco31I,Bso31I,BspTNI]
Cuts 1 time.
Cuts at position 5843.
BsaAI
(yAC'GTr)
[BstBAI]
Cuts 1 time.
Cuts at position 306.
BsaBI
(GATnn'nnATC)
[Bse8I,BseJI,BsiBI,BsrBRI,MamI]
[dam methylated]
Cuts 1 time.
Cuts at position [2287].
BsaXI
(ACnnnnnCTCCnnnnnnn_nnn')
Cuts 1 time.
Cuts at position 4734.
BsaXI
(GGAGnnnnnGTnnnnnnnnn_nnn')
Cuts 1 time.
Cuts at position 4764.
BseRI
(GAGGAGnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 730.
BsiWI
(C'GTAC_G)
[Pfl23II,PspLI,SunI]
Cuts 1 time.
Cuts at position 3727.
BspEI
(T'CCGG_A)
[AccIII,BseAI,BsiMI,Bsp13I,Kpn2I,MroI]
[dam methylated]
Cuts 1 time.
Cuts at position 617.
BssHII
(G'CGCG_C)
[BsePI,PauI]
Cuts 1 time.
Cuts at position 2454.
BstAPI
(GCAn_nnn'nTGC)
Cuts 1 time.
Cuts at position 6954.
BstZ17I
(GTA'TAC)
[BssNAI,Bst1107I]
Cuts 1 time.
Cuts at position 3721.
Bsu36I
(CC'TnA_GG)
[AocI,AxyI,Bse21I,CvnI,Eco81I]
Cuts 1 time.
Cuts at position 1180.
ClaI
(AT'CG_AT)
[BanIII,Bsa29I,BseCI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 1780.
DraIII
(CAC_nnn'GTG)
[AdeI]
Cuts 1 time.
Cuts at position 2146.
EcoRI
(G'AATT_C)
[FunII]
Cuts 1 time.
Cuts at position 1.
EcoRV
(GAT'ATC)
[Eco32I]
Cuts 1 time.
Cuts at position 2069.
FalI
(AAGnnnnnCTTnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 802.
HindIII
(A'AGCT_T)
Cuts 1 time.
Cuts at position 4530.
KasI
(G'GCGC_C)
[NarI,BbeI,EgeI,EheI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 7004.
MfeI
(C'AATT_G) [MunI]
Cuts 1 time.
Cuts at position 4446.
NarI
(GG'CG_CC)
[BbeI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 7005.
NcoI
(C'CATG_G)
[Bsp19I]
Cuts 1 time.
Cuts at position 326.
PpuMI
(rG'GwC_Cy)
[PpuXI,Psp5II,PspPPI]
[dcm methylated]
Cuts 1 time.
Cuts at position 702.
PsiI
(TTA'TAA)
Cuts 1 time.
Cuts at position 4417.
SalI
(G'TCGA_C)
Cuts 1 time.
Cuts at position 4512.
SapI
(GCTCTTCn'nnn_)
Cuts 1 time.
Cuts at position 4773.
ScaI
(AGT'ACT)
[Acc113I,AssI]
Cuts 1 time.
Cuts at position 6262.
SfoI
(GGC'GCC)
[NarI,BbeI,EgeI,EheI,KasI,Mly113I]
Cuts 1 time.
Cuts at position 7006.
SmaI
(CCC'GGG)
[Cfr9I,PspAI,XmaI,XmaCI]
Cuts 1 time.
Cuts at position 832.
SnaBI
(TAC'GTA)
[BstSNI,Eco105I]
Cuts 1 time.
Cuts at position 306.
StuI
(AGG'CCT)
[AatI,Eco147I,PceI,SseBI]
[dcm methylated]
Cuts 1 time.
Cuts at position 769.
StyI
(C'CwwG_G)
[BssT1I,Eco130I,EcoT14I,ErhI]
Cuts 1 time.
Cuts at position 326.
XhoI
(C'TCGA_G)
[BssHI,PaeR7I,Sfr274I,SlaI,StrI,TliI]
Cuts 1 time.
Cuts at position 640.
XmaI
(C'CCGG_G)
[SmaI,Cfr9I,PspAI,XmaCI]
Cuts 1 time.
Cuts at position 830.
XmnI
(GAAnn'nnTTC)
[Asp700I,MroXI,PdmI]
Cuts 1 time.
Cuts at position 6381.