pSV40b Mammalian lacZ Vector
Overview:
This common
eukaryotic expression vector, pSV40b expresses the full-length b-galactosidase gene under the control
of simian virus 40 (SV40) early
promoter. This vector is very useful
for transfection of mammalian cells in culture and for use in other
species. The b-galactosidase enzyme expression is enhanced
by elements including: SD/SA-RNA splice donor and acceptor sequence, and SV40
late polyadenylylation signal. pSV40b expression vector also contains b-lactamase, which acts as a selection marker
(100 mg/ml ampicillin resistance) in E. coli host. See also our pCMVb vector system, Product M0951.
Price: 20mg $99.75 (USD)
References:
- Hall, C.V. et al., 1983. “Expression
and Regulation of Escherichia coli lacZ Gene Fusions in Mammalian
Cells.” J. Mol. Appl. Gen. 2:101.
- Herbomel, P. et al., 1984. “Two
distinct enhancers with different cell specificities coexist in the
regulatory region of polyoma.”
Cell 39:653.
- Nolan, G.P., et al., 1988. “Fluorescence-activated
Cell Analysis and Sorting of Viable Mammalian Cells Based on b-D-galactosidase Activity after
Transduction of Escherichia coli lacZ.” Proc. Natl. Acad. Sci
(USA). 85:2603.
- Nikcevic, G. et al., 2003. “Improved
transfection efficiency of cultured human cells.” Cell Biol. Int. 27:735.