pSV40beta, 7045 bp. (CIRCULAR)
Restriction analysis 3/29/2004
pDRAW32 revision 1.1.74
Rebase containing 253 enzymes.
44 enzymes match enzyme selection criteria.
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AfeI
(AGC'GCT)
[Eco47III,Aor51HI,FunI]
Cuts 1 time.
Cuts at position 2672.
AhdI
(GACnn_n'nnGTC)
[Eam1105I,AspEI,DriI,EclHKI]
Cuts 1 time.
Cuts at position 5663.
AvrII
(C'CTAG_G)
[AspA2I,BlnI,XmaJI]
Cuts 1 time.
Cuts at position 381.
BaeI
(ACnnnnGTAyCnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 1061.
BaeI
(GrTACnnnnGTnnnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 1094.
BbeI
(G_GCGC'C)
[NarI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 6889.
BclI
(T'GATC_A)
[FbaI,Ksp22I]
[dam methylated]
Cuts 1 time.
Cuts at position [2183].
BglII
(A'GATC_T)
Cuts 1 time.
Cuts at position 517.
BlpI
(GC'TnA_GC)
[Bpu1102I,Bsp1720I,CelII]
Cuts 1 time.
Cuts at position 3850.
BplI
(GAGnnnnnCTCnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 348.
Bpu10I
(CC'TnA_GC)
Cuts 1 time.
Cuts at position 1342.
BsaI
(GGTCTCn'nnnn_)
[Eco31I,Bso31I,BspTNI]
Cuts 1 time.
Cuts at position 5724.
BsaBI
(GATnn'nnATC)
[Bse8I,BseJI,MamI]
[dam methylated]
Cuts 1 time.
Cuts at position [2168].
BsiWI
(C'GTAC_G)
[Pfl23II,PspLI,SunI]
Cuts 1 time.
Cuts at position 3608.
BssHII
(G'CGCG_C)
[BsePI,PauI]
Cuts 1 time.
Cuts at position 2335.
BstZ17I
(GTA'TAC)
[BssNAI,Bst1107I]
Cuts 1 time.
Cuts at position 3602.
Bsu36I
(CC'TnA_GG) [AxyI,Bse21I,Eco81I]
Cuts 1 time.
Cuts at position 1061.
ClaI
(AT'CG_AT)
[BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 1661.
DraIII
(CAC_nnn'GTG)
[AdeI]
Cuts 1 time.
Cuts at position 2027.
EcoICRI
(GAG'CTC)
[SacI,Ecl136II,Psp124BI,SstI]
Cuts 1 time.
Cuts at position 2775.
EcoRI
(G'AATT_C)
[FunII]
Cuts 1 time.
Cuts at position 1.
EcoRV
(GAT'ATC)
[Eco32I]
Cuts 1 time.
Cuts at position 1950.
HindIII
(A'AGCT_T)
Cuts 2 times.
KasI
(G'GCGC_C)
[NarI,BbeI,EgeI,EheI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 6885.
MfeI
(C'AATT_G) [MunI]
Cuts 1 time.
Cuts at position 4327.
NarI
(GG'CG_CC)
[BbeI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 6886.
NcoI
(C'CATG_G)
[Bsp19I]
Cuts 1 time.
Cuts at position 288.
NotI
(GC'GGCC_GC)
[CciNI]
Cuts 2 times.
PpuMI
(rG'GwC_Cy)
[PpuXI,Psp5II,PspPPI]
[dcm methylated]
Cuts 1 time.
Cuts at position 583.
PsiI
(TTA'TAA)
Cuts 1 time.
Cuts at position 4298.
PstI
(C_TGCA'G)
[BspMAI]
Cuts 1 time.
Cuts at position 4403.
SacI
(G_AGCT'C)
[Ecl136II,EcoICRI,Psp124BI,SstI]
Cuts 1 time.
Cuts at position 2777.
SalI
(G'TCGA_C)
Cuts 1 time.
Cuts at position 4393.
SapI
(GCTCTTCn'nnn_)
Cuts 1 time.
Cuts at position 4654.
SbfI
(CC_TGCA'GG)
[Sse8387I,SdaI]
Cuts 1 time.
Cuts at position 4403.
ScaI
(AGT'ACT) [ZrmI]
Cuts 1 time.
Cuts at position 6143.
SexAI
(A'CCwGG_T)
[MabI]
[dcm methylated]
Cuts 1 time.
Cuts at position [148].
SfiI
(GGCCn_nnn'nGGCC)
[dcm methylated]
Cuts 1 time.
Cuts at position 334.
SfoI
(GGC'GCC)
[NarI,BbeI,EgeI,EheI,KasI,Mly113I]
Cuts 1 time.
Cuts at position 6887.
SmaI
(CCC'GGG)
[Cfr9I,PspAI,XmaI,XmaCI]
Cuts 1 time.
Cuts at position 713.
XbaI
(T'CTAG_A)
[dam methylated]
Cuts 1 time.
Cuts at position 4387.
XhoI
(C'TCGA_G)
[BssHI,PaeR7I,Sfr274I,SlaI,TliI]
Cuts 1 time.
Cuts at position 521.
XmaI
(C'CCGG_G)
[SmaI,Cfr9I,PspAI,XmaCI]
Cuts 1 time.
Cuts at position 711.
XmnI
(GAAnn'nnTTC)
[Asp700I,MroXI,PdmI]
Cuts 1 time.
Cuts at position 6262.