pCMVbetalacZco, 6647 bp. (CIRCULAR)
Restriction analysis 9/8/2004
pDRAW32 revision 1.1.74
Rebase containing 253 enzymes.
62 enzymes match enzyme selection criteria.
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AarI (CACCTGCnnnn'nnnn_)
Cuts 1 time.
Cuts at position 3626.
AccI (GT'mk_AC) [FblI,XmiI]
Cuts 2 times.
AclI (AA'CG_TT) [Psp1406I]
Cuts 2 times.
AcuI (CTGAAGnnnnnnnnnnnnnn_nn') [Eco57I]
Cuts 2 times.
AfeI (AGC'GCT) [Eco47III,Aor51HI,FunI]
Cuts 2 times.
AhdI (GACnn_n'nnGTC) [Eam1105I,AspEI,DriI,EclHKI]
Cuts 2 times.
AleI (CACnn'nnGTG) [OliI]
Cuts 2 times.
AlfI (GCAnnnnnnTGCnnnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 1756.
ApaI (G_GGCC'C) [Bsp120I,PspOMI]
[dcm methylated]
Cuts 1 time.
Cuts at position [3400].
ApoI (r'AATT_y) [AcsI,XapI]
Cuts 2 times.
BanII (G_rGCy'C) [Eco24I,EcoT38I,FriOI]
Cuts 2 times.
BbsI (GAAGACnn'nnnn_) [BpiI,BpuAI,BstV2I]
Cuts 1 time.
Cuts at position 600.
BciVI (GTATCCnnnnn_n') [BfuI]
Cuts 2 times.
BglII (A'GATC_T)
Cuts 1 time.
Cuts at position 1358.
BplI (GAGnnnnnCTCnnnnnnnn_nnnnn')
Cuts 2 times.
BsaBI (GATnn'nnATC) [Bse8I,BseJI,MamI]
[dam methylated]
Cuts 2 times.
BseRI (GAGGAGnnnnnnnn_nn')
Cuts 2 times.
BsmI (GAATG_Cn') [BsaMI,Mva1269I,PctI]
Cuts 2 times.
BspEI (T'CCGG_A) [AccIII,BseAI,Bsp13I,Kpn2I,MroI]
[dam methylated]
Cuts 1 time.
Cuts at position 617.
BsrDI (GCAATG_nn') [Bse3DI,BseMI]
Cuts 2 times.
BsrGI (T'GTAC_A) [Bsp1407I,BstAUI,SspBI]
Cuts 1 time.
Cuts at position 3470.
BssHII (G'CGCG_C) [BsePI,PauI]
Cuts 1 time.
Cuts at position 1227.
BstAPI (GCAn_nnn'nTGC)
Cuts 1 time.
Cuts at position 6437.
BstEII (G'GTnAC_C) [BstPI,Eco91I,EcoO65I,PspEI]
Cuts 1 time.
Cuts at position 2607.
BstXI (CCAn_nnnn'nTGG)
Cuts 1 time.
Cuts at position 3297.
BtgZI (GCGATGnnnnnnnnnn'nnnn_)
Cuts 1 time.
Cuts at position 300.
ClaI (AT'CG_AT)
[BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 656.
DraIII (CAC_nnn'GTG) [AdeI]
Cuts 1 time.
Cuts at position 1898.
DrdI (GACnn_nn'nnGTC) [AasI,DseDI]
Cuts 2 times.
EcoICRI (GAG'CTC) [SacI,Ecl136II,Psp124BI,SstI]
Cuts 1 time.
Cuts at position 532.
EcoRI (G'AATT_C)
[FunII]
Cuts 1 time.
Cuts at position 1.
FseI (GG_CCGG'CC)
Cuts 1 time.
Cuts at position 2831.
FspAI (rTGC'GCAy)
Cuts 1 time.
Cuts at position 1702.
HindIII (A'AGCT_T)
Cuts 2 times.
HpaI (GTT'AAC) [KspAI]
Cuts 1 time.
Cuts at position 3920.
MfeI (C'AATT_G) [MunI]
Cuts 1 time.
Cuts at position 3929.
MscI (TGG'CCA) [BalI,MlsI,MluNI,Msp20I]
[dcm methylated]
Cuts 2 times.
NdeI (CA'TA_TG) [FauNDI]
Cuts 2 times.
NotI (GC'GGCC_GC) [CciNI]
Cuts 1 time.
Cuts at position 3776.
PfoI (T'CCnGG_A)
Cuts 1 time.
Cuts at position 6298.
PmlI (CAC'GTG) [PmaCI,AcvI,BbrPI,Eco72I,PspCI]
Cuts 1 time.
Cuts at position 1374.
PpuMI (rG'GwC_Cy) [PpuXI,Psp5II,PspPPI]
[dcm methylated]
Cuts 1 time.
Cuts at position 3450.
PsiI (TTA'TAA)
Cuts 1 time.
Cuts at position 3900.
PspOMI (G'GGCC_C) [ApaI,Bsp120I]
Cuts 1 time.
Cuts at position 3396.
PspXI (vC'TCGA_Gb)
Cuts 1 time.
Cuts at position 640.
PvuI (CG_AT'CG) [BspCI,Ple19I]
Cuts 2 times.
SacI (G_AGCT'C) [Ecl136II,EcoICRI,Psp124BI,SstI]
Cuts 1 time.
Cuts at position 534.
SacII (CC_GC'GG) [Cfr42I,KspI,Sfr303I,SgrBI]
Cuts 2 times.
SalI (G'TCGA_C)
Cuts 2 times.
SapI (GCTCTTCn'nnn_)
Cuts 1 time.
Cuts at position 4256.
ScaI (AGT'ACT) [ZrmI]
Cuts 2 times.
SfiI (GGCCn_nnn'nGGCC)
[dcm methylated]
Cuts 1 time.
Cuts at position [3367].
SgrAI (Cr'CCGG_yG)
Cuts 1 time.
Cuts at position 1231.
SnaBI (TAC'GTA) [BstSNI,Eco105I]
Cuts 1 time.
Cuts at position 306.
SphI (G_CATG'C) [BbuI,PaeI,SpaHI]
Cuts 2 times.
SspI (AAT'ATT)
Cuts 1 time.
Cuts at position 6069.
StuI (AGG'CCT) [AatI,Eco147I,PceI,SseBI]
[dcm methylated]
Cuts 2 times.
TfiI (G'AwT_C) [PfeI]
Cuts 2 times.
Tth111I (GACn'n_nGTC) [AspI,PflFI,PsyI,TelI]
Cuts 1 time.
Cuts at position 1467.
XcmI (CCAnnnn_n'nnnnTGG)
Cuts 2 times.
XhoI (C'TCGA_G) [BssHI,PaeR7I,Sfr274I,SlaI,TliI]
Cuts 1 time.
Cuts at position 640.
XmnI (GAAnn'nnTTC) [Asp700I,MroXI,PdmI]
Cuts 2 times.