pCMVbetalacznls12co, 6683 bp. (CIRCULAR)
Restriction analysis 9/8/2004
pDRAW32 revision 1.1.74
Rebase containing 253 enzymes.
62 enzymes match enzyme selection criteria.
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AarI
(CACCTGCnnnn'nnnn_)
Cuts 1 time.
Cuts at position 3662.
AccI
(GT'mk_AC)
[FblI,XmiI]
Cuts 2 times.
AclI
(AA'CG_TT)
[Psp1406I]
Cuts 2 times.
AcuI
(CTGAAGnnnnnnnnnnnnnn_nn') [Eco57I]
Cuts 2 times.
AfeI
(AGC'GCT)
[Eco47III,Aor51HI,FunI]
Cuts 2 times.
AhdI
(GACnn_n'nnGTC)
[Eam1105I,AspEI,DriI,EclHKI]
Cuts 2 times.
AleI
(CACnn'nnGTG)
[OliI]
Cuts 2 times.
AlfI
(GCAnnnnnnTGCnnnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 1792.
ApaI
(G_GGCC'C)
[Bsp120I,PspOMI]
[dcm methylated]
Cuts 1 time.
Cuts at position [3436].
ApoI
(r'AATT_y)
[AcsI,XapI]
Cuts 2 times.
BanII
(G_rGCy'C)
[Eco24I,EcoT38I,FriOI]
Cuts 2 times.
BbsI
(GAAGACnn'nnnn_)
[BpiI,BpuAI,BstV2I]
Cuts 1 time.
Cuts at position 600.
BciVI
(GTATCCnnnnn_n')
[BfuI]
Cuts 2 times.
BglII
(A'GATC_T)
Cuts 1 time.
Cuts at position 1394.
BplI
(GAGnnnnnCTCnnnnnnnn_nnnnn')
Cuts 2 times.
BsaBI
(GATnn'nnATC)
[Bse8I,BseJI,MamI]
[dam methylated]
Cuts 2 times.
BseRI
(GAGGAGnnnnnnnn_nn')
Cuts 2 times.
BsmI
(GAATG_Cn')
[BsaMI,Mva1269I,PctI]
Cuts 2 times.
BspEI
(T'CCGG_A)
[AccIII,BseAI,Bsp13I,Kpn2I,MroI]
[dam methylated]
Cuts 1 time.
Cuts at position 617.
BsrDI
(GCAATG_nn')
[Bse3DI,BseMI]
Cuts 2 times.
BsrGI
(T'GTAC_A)
[Bsp1407I,BstAUI,SspBI]
Cuts 1 time.
Cuts at position 3506.
BssHII
(G'CGCG_C)
[BsePI,PauI]
Cuts 1 time.
Cuts at position 1263.
BstAPI
(GCAn_nnn'nTGC)
Cuts 1 time.
Cuts at position 6473.
BstEII
(G'GTnAC_C)
[BstPI,Eco91I,EcoO65I,PspEI]
Cuts 1 time.
Cuts at position 2643.
BstXI
(CCAn_nnnn'nTGG)
Cuts 1 time.
Cuts at position 3333.
BtgZI
(GCGATGnnnnnnnnnn'nnnn_)
Cuts 1 time.
Cuts at position 300.
ClaI
(AT'CG_AT)
[BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 656.
DraIII
(CAC_nnn'GTG)
[AdeI]
Cuts 1 time.
Cuts at position 1934.
DrdI
(GACnn_nn'nnGTC)
[AasI,DseDI]
Cuts 2 times.
EcoICRI
(GAG'CTC)
[SacI,Ecl136II,Psp124BI,SstI]
Cuts 1 time.
Cuts at position 532.
EcoRI
(G'AATT_C)
[FunII]
Cuts 1 time.
Cuts at position 1.
FseI
(GG_CCGG'CC)
Cuts 1 time.
Cuts at position 2867.
FspAI
(rTGC'GCAy)
Cuts 1 time.
Cuts at position 1738.
HindIII
(A'AGCT_T)
Cuts 2 times.
HpaI
(GTT'AAC) [KspAI]
Cuts 1 time.
Cuts at position 3956.
MfeI
(C'AATT_G) [MunI]
Cuts 1 time.
Cuts at position 3965.
MscI
(TGG'CCA)
[BalI,MlsI,MluNI,Msp20I]
[dcm methylated]
Cuts 2 times.
NdeI
(CA'TA_TG)
[FauNDI]
Cuts 2 times.
NotI
(GC'GGCC_GC)
[CciNI]
Cuts 1 time.
Cuts at position 3812.
PfoI
(T'CCnGG_A)
Cuts 1 time.
Cuts at position 6334.
PmlI
(CAC'GTG)
[PmaCI,AcvI,BbrPI,Eco72I,PspCI]
Cuts 1 time.
Cuts at position 1410.
PpuMI
(rG'GwC_Cy)
[PpuXI,Psp5II,PspPPI]
[dcm methylated]
Cuts 2 times.
PsiI
(TTA'TAA)
Cuts 1 time.
Cuts at position 3936.
PspOMI
(G'GGCC_C)
[ApaI,Bsp120I]
Cuts 1 time.
Cuts at position 3432.
PspXI
(vC'TCGA_Gb)
Cuts 1 time.
Cuts at position 640.
PvuI
(CG_AT'CG)
[BspCI,Ple19I]
Cuts 2 times.
SacI
(G_AGCT'C)
[Ecl136II,EcoICRI,Psp124BI,SstI]
Cuts 1 time.
Cuts at position 534.
SacII
(CC_GC'GG)
[Cfr42I,KspI,Sfr303I,SgrBI]
Cuts 2 times.
SalI
(G'TCGA_C)
Cuts 2 times.
SapI
(GCTCTTCn'nnn_)
Cuts 1 time.
Cuts at position 4292.
ScaI
(AGT'ACT) [ZrmI]
Cuts 2 times.
SfiI
(GGCCn_nnn'nGGCC)
[dcm methylated]
Cuts 1 time.
Cuts at position [3403].
SgrAI
(Cr'CCGG_yG)
Cuts 1 time.
Cuts at position 1267.
SnaBI
(TAC'GTA)
[BstSNI,Eco105I]
Cuts 1 time.
Cuts at position 306.
SphI
(G_CATG'C)
[BbuI,PaeI,SpaHI]
Cuts 2 times.
SspI
(AAT'ATT)
Cuts 1 time.
Cuts at position 6105.
StuI
(AGG'CCT)
[AatI,Eco147I,PceI,SseBI]
[dcm methylated]
Cuts 2 times.
TfiI
(G'AwT_C) [PfeI]
Cuts 2 times.
Tth111I
(GACn'n_nGTC)
[AspI,PflFI,PsyI,TelI]
Cuts 1 time.
Cuts at position 1503.
XcmI
(CCAnnnn_n'nnnnTGG)
Cuts 2 times.
XhoI
(C'TCGA_G)
[BssHI,PaeR7I,Sfr274I,SlaI,TliI]
Cuts 1 time.
Cuts at position 640.
XmnI
(GAAnn'nnTTC)
[Asp700I,MroXI,PdmI]
Cuts 2 times.