pSV40betalacznls12co, 6564 bp. (CIRCULAR)
Restriction analysis 9/8/2004
pDRAW32 revision 1.1.74
Rebase containing 253 enzymes.
58 enzymes match enzyme selection criteria.
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AarI
(CACCTGCnnnn'nnnn_)
Cuts 1 time.
Cuts at position 3543.
AatII
(G_ACGT'C) [ZraI]
Cuts 1 time.
Cuts at position 6104.
AccI
(GT'mk_AC)
[FblI,XmiI]
Cuts 2 times.
AclI
(AA'CG_TT)
[Psp1406I]
Cuts 2 times.
AcuI
(CTGAAGnnnnnnnnnnnnnn_nn') [Eco57I]
Cuts 2 times.
AfeI
(AGC'GCT)
[Eco47III,Aor51HI,FunI]
Cuts 2 times.
AhdI
(GACnn_n'nnGTC)
[Eam1105I,AspEI,DriI,EclHKI]
Cuts 2 times.
AleI
(CACnn'nnGTG)
[OliI]
Cuts 2 times.
ApaI
(G_GGCC'C)
[Bsp120I,PspOMI]
[dcm methylated]
Cuts 1 time.
Cuts at position [3317].
ApoI
(r'AATT_y)
[AcsI,XapI]
Cuts 2 times.
AvrII
(C'CTAG_G)
[AspA2I,BlnI,XmaJI]
Cuts 1 time.
Cuts at position 381.
BanII
(G_rGCy'C)
[Eco24I,EcoT38I,FriOI]
Cuts 1 time.
Cuts at position 3317.
BciVI
(GTATCCnnnnn_n')
[BfuI]
Cuts 2 times.
BfrBI
(ATG'CAT)
[EcoT22I,Mph1103I,NsiI,Zsp2I]
Cuts 2 times.
BglII
(A'GATC_T)
Cuts 2 times.
BsaBI
(GATnn'nnATC)
[Bse8I,BseJI,MamI]
[dam methylated]
Cuts 2 times.
BsmI
(GAATG_Cn')
[BsaMI,Mva1269I,PctI]
Cuts 2 times.
BsmBI
(CGTCTCn'nnnn_)
[Esp3I]
Cuts 2 times.
BsrDI
(GCAATG_nn')
[Bse3DI,BseMI]
Cuts 2 times.
BsrGI
(T'GTAC_A)
[Bsp1407I,BstAUI,SspBI]
Cuts 1 time.
Cuts at position 3387.
BssHII
(G'CGCG_C)
[BsePI,PauI]
Cuts 1 time.
Cuts at position 1144.
BstEII
(G'GTnAC_C)
[BstPI,Eco91I,EcoO65I,PspEI]
Cuts 1 time.
Cuts at position 2524.
BstXI
(CCAn_nnnn'nTGG)
Cuts 1 time.
Cuts at position 3214.
ClaI
(AT'CG_AT)
[BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 537.
DraIII
(CAC_nnn'GTG)
[AdeI]
Cuts 1 time.
Cuts at position 1815.
DrdI
(GACnn_nn'nnGTC)
[AasI,DseDI]
Cuts 2 times.
EcoRI
(G'AATT_C)
[FunII]
Cuts 1 time.
Cuts at position 1.
FalI
(AAGnnnnnCTTnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 432.
FseI
(GG_CCGG'CC)
Cuts 1 time.
Cuts at position 2748.
FspAI
(rTGC'GCAy)
Cuts 1 time.
Cuts at position 1619.
HpaI
(GTT'AAC) [KspAI]
Cuts 1 time.
Cuts at position 3837.
MfeI
(C'AATT_G) [MunI]
Cuts 1 time.
Cuts at position 3846.
MscI
(TGG'CCA)
[BalI,MlsI,MluNI,Msp20I]
[dcm methylated]
Cuts 2 times.
NdeI
(CA'TA_TG)
[FauNDI]
Cuts 1 time.
Cuts at position 6353.
NotI
(GC'GGCC_GC)
[CciNI]
Cuts 1 time.
Cuts at position 3693.
NsiI
(A_TGCA'T)
[BfrBI,EcoT22I,Mph1103I,Zsp2I]
Cuts 2 times.
PfoI
(T'CCnGG_A)
Cuts 1 time.
Cuts at position 6215.
PmlI
(CAC'GTG)
[PmaCI,AcvI,BbrPI,Eco72I,PspCI]
Cuts 1 time.
Cuts at position 1291.
PpuMI
(rG'GwC_Cy)
[PpuXI,Psp5II,PspPPI]
[dcm methylated]
Cuts 2 times.
PsiI
(TTA'TAA)
Cuts 1 time.
Cuts at position 3817.
PspOMI
(G'GGCC_C)
[ApaI,Bsp120I]
Cuts 1 time.
Cuts at position 3313.
PvuI
(CG_AT'CG)
[BspCI,Ple19I]
Cuts 2 times.
SacII
(CC_GC'GG)
[Cfr42I,KspI,Sfr303I,SgrBI]
Cuts 2 times.
SalI
(G'TCGA_C)
Cuts 2 times.
SapI
(GCTCTTCn'nnn_)
Cuts 1 time.
Cuts at position 4173.
SbfI
(CC_TGCA'GG)
[Sse8387I,SdaI]
Cuts 2 times.
ScaI
(AGT'ACT) [ZrmI]
Cuts 2 times.
SexAI
(A'CCwGG_T)
[MabI]
[dcm methylated]
Cuts 1 time.
Cuts at position [148].
SfiI
(GGCCn_nnn'nGGCC)
[dcm methylated]
Cuts 2 times.
SgrAI
(Cr'CCGG_yG)
Cuts 1 time.
Cuts at position 1148.
SspI
(AAT'ATT)
Cuts 1 time.
Cuts at position 5986.
TfiI
(G'AwT_C) [PfeI]
Cuts 2 times.
Tth111I
(GACn'n_nGTC)
[AspI,PflFI,PsyI,TelI]
Cuts 1 time.
Cuts at position 1384.
XbaI
(T'CTAG_A)
[dam methylated]
Cuts 2 times.
XcmI
(CCAnnnn_n'nnnnTGG)
Cuts 2 times.
XhoI
(C'TCGA_G)
[BssHI,PaeR7I,Sfr274I,SlaI,TliI]
Cuts 1 time.
Cuts at position 521.
XmnI
(GAAnn'nnTTC)
[Asp700I,MroXI,PdmI]
Cuts 2 times.
ZraI
(GAC'GTC) [AatII]
Cuts 1 time.
Cuts at position 6102.