Beta-Glucuronidase (GUS)

The β-glucuronidase (GUS) enzyme from E. coli has been well documented to provide desirable characteristics as a marker gene in transformed plants.

The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport.

Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule. This allows for  high sensitivity fluorometric, and spectrophotometric measurements of β-glucuronidase gene fusion expression.

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  1. M5215 large
    Isopropyl-beta-D-thioglucuronic acid, sodium salt
    $183.00

    Product size: 0.5 g

    lacZ inducer Learn More

    Product ID: M5215

  2. MarkerGene™ Live:Dead/Cytotoxicity Assay Kit, Blue/Green Staining
    MarkerGene™ Live:Dead/Cytotoxicity Assay Kit, Blue/Green Staining
    $189.00

    Product size: 1 kit

    This kit allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity (live cells) with a blue emitting dye while staining of nucleic acids in chromatin (dead cells) with a green emitting dye. Learn More

    Product ID: M1811

  3. 6-Chloro-indolyl β-D-glucuronic acid, lithium salt
    6-Chloro-indolyl β-D-glucuronic acid, lithium salt
    $89.00

    Product size: 10mg

    Chromogenic substrate for β-D-Glucuronidase(GUS). Learn More

    Product ID: M0254

  4. pCambia2301 Plant Expression Vector
    pCambia2301 Plant Expression Vector
    $144.00

    Product size: 20ug

    Vector contains genes for bacterial and plant selection with kanamycin and the gusA reporter gene. Learn More

    Product ID: M1710

  5. pCambia1391Z Plant Expression Vector
    pCambia1391Z Plant Expression Vector
    $144.00

    Product size: 20ug

    Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the gusA reporter gene and the lacZ gene for white/blue selection. Learn More

    Product ID: M1707

  6. pCambia1391Xc Plant Expression Vector
    pCambia1391Xc Plant Expression Vector
    $144.00

    Product size: 20ug

    Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame c. Learn More

    Product ID: M1706

  7. pCambia1391Xa Plant Expression Vector
    pCambia1391Xa Plant Expression Vector
    $144.00

    Product size: 20ug

    Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame a. Learn More

    Product ID: M1705

  8. pCambia1391 Plant Expression Vector
    pCambia1391 Plant Expression Vector
    $144.00

    Product size: 20ug

    Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the gusA reporter gene. Learn More

    Product ID: M1704

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