Calcium Green™, hexapotassium salt - equivalent

Product ID:
M0401
Unit Size
0.5 mg
Please order via www.abcam.com
Contact us for Availability
Description
Cell-impermeant, quantitative Calcium ion indicator with high affinity and low background fluorescence.
Calcium Green™ (EX 490nm / EM 531 nm) has been widely used for measurements of Calcium ion concentrations in live cells and tissues. Although Calcium Green™ is structurally similar to another popular calcium ion indicator, Fluo-3, it is more fluorescent at low calcium concentrations (Kd = 190 uM), facilitating the determination of baseline Ca2+ levels and increasing monitoring in resting cells. It has a high quantum yield with low phototoxicity and can be imaged in virtually all fluorescent microscopes. It’s fluorescence emission increases approximately 100 fold upon binding Ca2+ with virtually no auto fluorescence. Calcium Green™ is also about 5-fold brighter than Fluo-3 at saturating Ca2+ levels so it can be used at much lower concentrations, which also reduces problems with phototoxicity. Calcium Green™ is a trademark of Thermo-Fisher Scientific. Inc.
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Product Data
Product ID M0401
CAS Number 154719-40-1
Unit Size 0.5 mg
Availability Contact us for Availability
Alternative Names Glycine, N-[2-[2-[2-[bis(carboxymethyl)amino]-5-[[2',7'-dichloro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene)-5-yl]carbonyl]amino]phenoxy]ethoxy]phenyl]-N-(carboxymethyl)-, hexapotassium salt
Absorption 490
Emission Wavelength 531
Detection Method Fluorescence
Molecular Formula C43H27Cl2K6N3O16
Molecular Weight 1147.19
Soluble In DMSO
Storage Conditions F, D
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References
  • Paredes RM, Etzler JC, Watts LT, Zheng W, Lechleiter JD. (2008) "Chemical calcium indicators." Methods 46(3):143-151.
  • Petit L, Maier E, Gibert M, Popoff MR, Benz R. (2001) "Clostridium perfringens epsilon toxin induces a rapid change of cell membrane permeability to ions and forms channels in artificial lipid bilayers." J Biol. Chem. 276(19):15736-15740.
  • Stricker SA. (2000) "Confocal microscopy of intracellular calcium dynamics during fertilization." Biotechniques 29(3):492-494.
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