DyRect™ Live-Cell Neutral Lipid Imaging Kit

Product ID:
M2675
Unit Size
1 kit
Please order via www.abcam.com
In Stock
Description
This easy to use kit fluorescently labels both neutral and polar lipids in live cells. When stained with this imaging kit, neutral lipids will fluoresce green while polar lipids like phospholipids will fluoresce red. Any free dye not associated with lipid remains non-fluorescent. Can by used with flow cytometry or fluorescence microscopy.
Foam cells and lipid engorged macrophages have been found to be prime suspects in the progression of various disease states, from Alzheimer’s to atherosclerosis. They are characterized by the accumulation of intracellular neutral lipid droplets from ingesting excess lipoproteins, giving them a foam-like appearance. The intracellular fat vacuoles contain neutral lipids like cholesterol, lipoproteins and triglycerides. The DyRectTM Live-Cell Neutral Lipid Imaging Kit (M2675) that can be used to label and analyze cellular lipids. When stained with our imaging kit, neutral lipids will fluoresce green (EX 485 nm/EM 525 nm) while polar lipids like phospholipids will fluoresce red (EX552 nm/EM636 nm). Any free dye not associated with lipid remains non-fluorescent. The environmentally sensitive probe used in this staining method can be added directly to live cell cultures to label the fat vacuoles within the cells and can subsequently be analyzed by flow cytometry or fluorescence microscopy.
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Product Data
Product ID M2675
Unit Size 1 kit
Availability In Stock
Emission Wavelength Red EX/EM: 552 nm/636 nm; Green EX/EM: 485 nm/525 nm
Detection Method Fluorescence
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References
  • Gerrity, RG (1981) “The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions.” The American journal of pathology, 103(2), p.181.
  • Casserly I, Topol EJ (2004) “Convergence of atherosclerosis and Alzheimer's disease: inflammation, cholesterol, and misfolded proteins.” The Lancet. 2004 Apr 3;363(9415):1139-1146.
  • Chen W, Jin F, Cao G, Mei R, Wang Y, Long P, Wang X, Ge W (2018) “ApoE4 May be a Promising Target for Treatment of Coronary Heart Disease and Alzheimer's Disease.” Current drug targets. 2018 Jul 1;19(9):1038-1044.
  • Werner T, Liebisch G, Grandl M, Schmitz G (2006) "Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages." Cytometry A (69):3 200-202.
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