Luciferase

A variety of species have evolved the ability to emit light biochemically. Among those that have found the most use in molecular biology applications are the North American firefly photinus pyralis, the marine copepod Gaussia princeps and the bacterium Vibrio harveyi. The firefly luciferase gene (luc) has been cloned into a variety of species, becoming a popular marker gene for plants, bacteria and mammalian cells. Measurement of transciptional regulation by coupling the expression of a reporter gene is widely used to monitor a variety of physiological events including the action of specific receptor elements on gene expression, signal transduction, expression of transcription factors, protein-protein interactions, viral infection and processing. The firefly luciferase gene encodes a functional protein immediately upon transcription, and an assay for the resulting light production is highly sensitive (very low background), rapid, and reliable, making luciferase a popular choice for reporter cloning experiments. These assays are also well suited to automation and high-throughput applications. The substrate, coelenterazine is common to a number of marine bioluminescent reactions, including those from Renilla, Gaussia, Aequorea and Watesenia. In some of these reactions it is utilized as a simple substrate being catalytically turned over in the bioluminescent reaction, while in others, such as in the photoprotein systems of Mneiopsis, it is incorporated as part of the photoprotein. The substrate h-coelenterate luciferin is a coelenterazine luciferin analog wherein one of the phenolic groups is replaced with a proton. This substrate exhibits a high recharging efficiency and even higher light output., fluorometric, and spectrophotometric measurements of β-glucuronidase gene fusion expression.

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  1.   pTA-p53-luc  Mammalian Transcription Factor Reporting Vector  with Red Luciferase
    pTA-p53-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase
    $361.00

    Product size: 20 ug

    A plasmid expression vector for monitoring p53 activation via luciferase in mammalian cells. Learn More

    Product ID: M2740

  2. pTA-NfκB-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase
    pTA-NfκB-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase
    $361.00

    Product size: 20 ug

    A plasmid expression vector for monitoring NfκB activation via luciferase in mammalian cells. Learn More

    Product ID: M2739

  3. MarkerGene™ Cell Proximity Assay Kit
    MarkerGene™ Cell Proximity Assay Kit
    $274.00

    Product size:

    Easy to use kit to determine cell-cell interactions and proximity. Learn More

    Product ID: M2751

  4. MarkerGene™ Bacterial Gram Staining Kit
    MarkerGene™ Bacterial Gram Staining Kit
    $133.00

    Product size: 1 kit

    This kit provides all the reagents and protocols for Gram staining of bacteria. Learn More

    Product ID: M2742

  5. pFHluc Mammalian Luciferase Expression Vector with Multiple Cloning Site
    pFHluc Mammalian Luciferase Expression Vector with Multiple Cloning Site
    $316.00

    Product size: 20ug

    High copy number eukaryotic vector, pFHluc, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Contains a multiple cloning site with 11 unique restriction sites. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:619) from D-luciferin. Learn More

    Product ID: M1796

  6. pDC99 Mammalian Luciferase Expression Vector
    pDC99 Mammalian Luciferase Expression Vector
    $194.00

    Product size: 20ug

    High copy number eukaryotic vector, pDC99 expresses a mutant luciferase gene under the control of the Simian virus 40Early Core (SV40) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. Learn More

    Product ID: M1395

  7. pDC57 Mammalian Luciferase Expression Vector
    pDC57 Mammalian Luciferase Expression Vector
    $194.00

    Product size: 20ug

    This high copy number eukaryotic vector, pDC57, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. Learn More

    Product ID: M1394

  8. pCdgCAT Mammalian CAT Expression Vector
    pCdgCAT Mammalian CAT Expression Vector
    $136.00

    Product size: 20μg

    High copy number eukaryotic vector, pCdgCAT expresses the E. coli Chloramphenicol Acetyltransferase (CAT) gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species. Learn More

    Product ID: M1327

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