MarkerGene™ ERLive™ β-Glucosidase Assay Kit

Product ID:
M3102
Unit Size
1 kit
Please order via www.abcam.com
In Stock
Description
This kit utilizes the sensitive substrate GlucRed to monitor beta-glucosidase activity in the Endoplasmic Reticulum (ER) of live cells.
 

The Endoplasmic Reticulum (ER) and Golgi Apparatus are dynamic organelles that contain metabolic and regulatory enzymes involved in the ordered synthesis and processing of nearly all proteins, lipids, and carbohydrates in the cell. Functional deficiencies or mutations in these enzymes are indicative of a number of disease states, particularly those that affect the nervous system including the Lysosomal Storage Diseases and Parkinson's Disease.

Methods to analyze ER enzyme function within living cells have been hampered by the inability to monitor activity within individual organelles. Our new ERLive™ organelle-targeted, live-cell fluorogenic substrates for ER enzymes can be used for in situ analysis of changes in metabolic activity in a live-cell format.  These new assays can be used for monitoring the activity of ER enzymes for such diseases as Parkinson's Disease, ALS and the Lysosomal Storage Diseases.  Staining with new new probes was shown to co-localize with known markers of ER and Golgi morphology. 

The beta-glucocerebrosidase enzyme is currently indicated to be involved in diseases such as Gaucher and Parkinson’s diseases. MarkerGene™ ERLive™ beta-Glucosidase Assay Kit utilizes a substrate which only becomes fluorescent upon cleavage by the enzyme in the ER where GCase beta-Glucocerebrosidase (GBA) activity will exhibit increased staining in a manner proportional to the enzyme activity levels being trapped by ER Stress events in the cell. 

Important Licensing Information: This product is covered by one or more United States or International Patents and is subject to our Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses. See product information sheet for more details
Corporate entity: Marker Gene Technologies, Inc., Eugene, Oregon, USA
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Publications Using This Product
  • Naleway JJ, Woods LC, Harlan FK,Swanson EM (2019) "Live-cell analysis of ER and Golgi Enzyme Activities in Gaucher Disease" Mol. Biol. Cell 30(26): 3075-3160.
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    Product Data
    Product ID M3102
    Unit Size 1 kit
    Availability In Stock
    Alternative Names GlucRed
    Absorption 572
    Emission Wavelength 585
    Extinction 37K
    Detection Method Fluorescence
    Soluble In DMSO
    Storage Conditions F, D
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    References
  • Westbroek W, Gustafson AM, Sidransky E. (2011) “Exploring the link between glucocerebrosidase mutations and parkinsonism.” Trends Mol Med. 17(9):485-493.
  • Sidransky E. (2005) “Gaucher disease and parkinsonism.” Mol. Genet. Metab. 84:302-304.
  • Sidransky E (2004) "Gaucher disease: complexity in a 'simple' disorder." Molecular genetics and metabolism 83(1-2):6-15.
  • Pap EHW, Dansen TB, van Summeren R, Wirtz KWA. (2001) “Peptide-based targeting of fluorophores to organelles in living cells.” Experimental Cell Research 265: 288-293.
  • Karageorgos LE, Isaac EL, Brooks DA, Ravenscroft EM, Davey R, Hopwood JJ, Meikle PJ. (1997) “Lysosomal biogenesis in lysosomal storage disorders.” Experimental Cell Research 234, 85-97.
  • Neudorfer O, Giladi N, Elstein D, Abrahamov A. (1996) "Occurrence of Parkinson’s syndrome in type 1 Gaucher disease." Qjm 691-694.
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