MarkerGene™ ERLive™ β-Glucosidase Assay Kit
The Endoplasmic Reticulum (ER) and Golgi Apparatus are dynamic organelles that contain metabolic and regulatory enzymes involved in the ordered synthesis and processing of nearly all proteins, lipids, and carbohydrates in the cell. Functional deficiencies or mutations in these enzymes are indicative of a number of disease states, particularly those that affect the nervous system including the Lysosomal Storage Diseases and Parkinson's Disease.
Methods to analyze ER enzyme function within living cells have been hampered by the inability to monitor activity within individual organelles. Our new ERLive™ organelle-targeted, live-cell fluorogenic substrates for ER enzymes can be used for in situ analysis of changes in metabolic activity in a live-cell format. These new assays can be used for monitoring the activity of ER enzymes for such diseases as Parkinson's Disease, ALS and the Lysosomal Storage Diseases. Staining with new new probes was shown to co-localize with known markers of ER and Golgi morphology.
The beta-glucocerebrosidase enzyme is currently indicated to be involved in diseases such as Gaucher and Parkinson’s diseases. MarkerGene™ ERLive™ beta-Glucosidase Assay Kit utilizes a substrate which only becomes fluorescent upon cleavage by the enzyme in the ER where GCase beta-Glucocerebrosidase (GBA) activity will exhibit increased staining in a manner proportional to the enzyme activity levels being trapped by ER Stress events in the cell.
|Unit Size||1 kit|
|Storage Conditions||F, D|
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