Opti-Klear™ Live Cell Imaging Buffer

Product ID:
M1898
Unit Size
30mL/5X concentrate
$48.00
  • Buy 5 for $38.40 each and save 20%
In stock
Description
HEPES based imaging buffer formulated to lower background fluorescence, maintain proper pH and osmolarity for hours at 37°, provide an energy source and preserve fluorescent signals. Shipped as 5X sterile filtered concentrate.
An ideal substitute for growth media for all extended live cell imaging sessions.
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Publications Using This Product
  • Kwee E, Saidel G, Powell K, Heylman C, Boehm C, Muschler G. (2019) "Quantifying proliferative and surface marker heterogeneity in colony-founding connective tissue progenitors and their progeny using time-lapse microscopy. J Tissue Eng Regen Med. 13(2):203-216.
  • Tomlinson RE, Li Z, Li Z, Minichiello L, Riddle RC, Venkatesan A, Clemens TL. (2017) "NGF-TrkA signaling in sensory nerves is required for skeletal adaptation to mechanical loads in mice." Proc Natl Acad Sci U S A. 2017 Apr 17. pii: 201701054.
  • Abshire JR, Rowlands CJ, Ganesan SM, So PT, Niles JC. (2017) "Quantification of labile heme in live malaria parasites using a genetically encoded biosensor." Proc Natl Acad Sci U S A. pii: 201615195.
  • Kwee E, Powell K, Muschler G (2015) "Characterization of connective tissue progenitors through phase contrast and multicolor fluorescence time-lapse microscopy ", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280O
  • Goldfless SJ, Wagner JC, Niles JC. (2014) "Versatile control of Plasmodium falciparum gene expression with an inducible protein-RNA interaction." Nat Commun. 5:5329.
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Product Data
Product ID M1898
Unit Size 30mL/5X concentrate
Availability In Stock
Storage Conditions 2-8C, Protect From Light
Notes “Yes -- we used it. It greatly reduces background -- I have already told a number of colleagues about it. Since it is 5x, a bottle can last for a while.” Dr. Aryeh Weiss, Faculty of Engineering Bar Ilan University, Israel
Fisher Scientific 50-447-704
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References
  • Diaz G, Isola R,Falchi A, Diana A. (1999) “CO2-Enriched Atmosphere on the Microscope Stage.” BioTechniques 27:292-294.
  • Spierenburg GT, Oerlemans FT, van Laarhoven JP, de Bruyn CH. (1984) “Phototoxicity of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered culture media for human leukemic cell lines.” Cancer Res. 1984 May;44(5):2253-4.
  • Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I. (1985) “Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.” In Vitro Cell Dev Biol. 21(5):282-7.
  • Lepe-Zuniga JL, Zigler JS Jr, Gery I. (1987) “Toxicity of light-exposed Hepes media.” J Immunol Methods. 103(1):145
  • Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. (1986) “Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.” Proc Natl Acad Sci U S A. 83(8):2496-500.
  • Lelong IH, Rebel G. (1998) “pH drift of "physiological buffers" and culture media used for cell incubation during in vitro studies.” J Pharmacol Toxicol Methods 39(4):203-10.
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