Reporter Assays make use of detectable nonendogenous genes to elucidate cellular processes. Many of these marker genes code for proteins that are easy to detect including enzymes such as lacZ β-galactosidase in mammalian cells, β-glucuronidase in plant cells, firefly and Gaussia luciferase, chloramphenicol transferase (CAT) as well as various fluorescent proteins. Marker Gene provides easy to use kits for detection of cloned marker gene expression as well as numerous vectors containing the marker gene for use in subcloning and monitoring transfection efficiency. Analysis of marker gene activity can be made with microtiterplate assay, flow cytometry, fluorescence activated cell sorting, fluorescence microscopy, histochemical / chomogenic staining methods or by cell lysis assay techniques. Systems are available to employ and monitor marker gene activities in mammalian, plant, bacterial, yeast and fungal cell lines.
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- Isopropyl-beta-D-thiogalactopyranoside, <5ppm dioxane Please order via www.abcam.com
- C12FDG (5-Dodecanoylaminofluorescein di-β-D-galactopyranoside) Please order via www.abcam.com
- pTA-p53-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase Please order via www.abcam.com
- pTA-NfκB-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase Please order via www.abcam.com