Reporter Assays

Reporter Assays

Reporter Assays make use of detectable nonendogenous genes to elucidate cellular processes. Many of these marker genes code for proteins that are easy to detect including enzymes such as lacZ β-galactosidase in mammalian cells, β-glucuronidase in plant cells, firefly and Gaussia luciferase, chloramphenicol transferase (CAT) as well as various fluorescent proteins. Marker Gene provides easy to use kits for detection of cloned marker gene expression as well as numerous vectors containing the marker gene for use in subcloning and monitoring transfection efficiency. Analysis of marker gene activity can be made with microtiterplate assay, flow cytometry, fluorescence activated cell sorting, fluorescence microscopy, histochemical / chomogenic staining methods or by cell lysis assay techniques. Systems are available to employ and monitor marker gene activities in mammalian, plant, bacterial, yeast and fungal cell lines. 

Choose a category at left or use the Search Engine above to find out more about these assays and systems for your specific application.

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  1. Isopropyl-beta-D-thiogalactopyranoside,
    Isopropyl-beta-D-thiogalactopyranoside, <5ppm dioxane
    $37.00

    Product size: 0.5 g

    inducer of protein expression Learn More

    Product ID: M5213

  2. M5284 large
    Phenylethyl-beta-D-thiogalactopyranoside
    $380.00

    Product size: 1g

    Cell-permeant competitive reversible inhibitor of beta-galactosidase activity. Learn More

    Product ID: M5284

  3. M5215 large
    Isopropyl-beta-D-thioglucuronic acid, sodium salt
    $183.00

    Product size: 0.5 g

    lacZ inducer Learn More

    Product ID: M5215

  4. M5214 large
    Isopropyl-beta-D-thioglucopyranoside
    $160.00

    Product size: 50 mg

    lacZ inducer Learn More

    Product ID: M5214

  5. C12FDG (5-Dodecanoylaminofluorescein di-β-D-galactopyranoside)
    C12FDG (5-Dodecanoylaminofluorescein di-β-D-galactopyranoside)
    $330.00

    Product size: 5mg

    Lipophilic Β-galactosidase substrate. Learn More

    Product ID: M2888

  6.   pTA-p53-luc  Mammalian Transcription Factor Reporting Vector  with Red Luciferase
    pTA-p53-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase
    $361.00

    Product size: 20 ug

    A plasmid expression vector for monitoring p53 activation via luciferase in mammalian cells. Learn More

    Product ID: M2740

  7. pTA-NfκB-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase
    pTA-NfκB-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase
    $361.00

    Product size: 20 ug

    A plasmid expression vector for monitoring NfκB activation via luciferase in mammalian cells. Learn More

    Product ID: M2739

  8. Acridine Orange
    Acridine Orange
    $20.00

    Product size: 10 mg

    Acridine Orange is a lysomotropic probe that can also be used for analysis of autophagy and to label DNA in living cells. Learn More

    Product ID: M1851

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