RubyGlow™ Luminescent Bacterial Detection Kit

Product ID:
M1573
Unit Size
1kit
$146.00
In Stock
Description
Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) to easily detect and quantify bacterial cells based upon the generation of ATP in metabolically active cells.
Each kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format. Bacteria from a number of media or sources can be detected with an extremely high sensitivity (low detection limit). In addition, extended incubation (2-4 hours) of samples at 37ºC can significantly lower the detection limit and increase sensitivity using a protocol that can be completed in one day. Assay is also amenable to high throughput (HTS) applications for analysis of bacterial susceptibility and antibiotic screening.
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Product Data
Product ID M1573
Unit Size 1kit
Availability In Stock
Emission Wavelength 619
Detection Method Luminescence
FAQS
⚛ Question: What is the detection limit? In particular, the number of bacteria in a 1mL sample or the quantity of ATP in a 1mL sample.

The assay measures the ATP produced by the bacteria. Most bacterial cells contain approx. 2x10-18 mol ATP per cell. Light emission is linear within a range of approx. 10 fg to 10 ng. The exact limits of the detection range will depend, of course, on the measuring device used, but theoretically this amount of ATP is similar to detection of about 1 bacterium. (10 fg = 10-14 gram and MW ATP = 507).

⚛ Question: Can we perform life / dead determination of bacteria in the sample?

Yes, you can use this assay to measure live:dead counts. You may wish to use some method to count “dead” cells independently (i.e. see our Kit M1891 for example), but the protocol describes the M1573 kit for ultrasensitive use in measuring bacterial counts after treatment with certain antibiotics, for example.

⚛ Question: Our culture media contains lipids. Will the lipids interfere with the assay?

Lipids in low concentrations should not affect the assay. Nonetheless, it will be good to run a control with and without lipid just to ensure any effect.

VWR International 101743-072
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References
  • Sherlock O, O'Connell N, Creamer E, Humphreys H. (2009) "Is it really clean? An evaluation of the efficacy of 4 methods for determining hospital cleanliness." J Hosp Infect 72:140–146.
  • Schondelmeyer CW, Dillehay DL, Webb SK, Huerkamp MJ, Mook DM, Pullium JK. (2006) "Investigation of appropriate sanitization frequency for rodent caging accessories: evidence supporting less-frequent cleaning." J Am Assoc Lab Anim Sci 45:40–43.
  • Sharpe AN, Woodrow MN, Jackson AK (1970) “Adenosine triphosphate (ATP) levels in foods contaminated by bacteria”. J. Appl. Bacteriol. 33: 758-767
  • Stannard CJ, Wood JM (1983) “The rapid estimation of microbial contamination of raw meat by measurement of adenosine triphosphate (ATP)”. J. Appl. Bacteriol. 55:429-438
  • Stanley PE (1986) “Extraction of adenosine triphosphate from microbial and somatic cells”. Methods Enzymol. 133:14-22
  • Karl DM (1980) “Cellular nucleotide measurement and applications in microbial ecology”. Microbiol. Rev. 44:739-96
  • McElroy WD, DeLuca MA (1983) “Firefly and bacterial luminescence: Basic science and applications”. J. Applied Biochem. 5:197-209
  • Ulrich PG, Wannlund JC (1984) “Bioluminescence in the microbiology laboratory”. American Clinical Products Rev.
  • Tuncan EU, Martin SE (1987) “Lysostaphin lysis procedure for detection of Staphylococcus aureus by the firefly bioluminescent ATP method”. Applied and Environmental Microbiology 53: 88-91
  • Lundin A, Thore A, (1975) “Analytical information obtainable by evaluation of the time course of firefly bioluminescence in the assay of ATP”. Anal. Biochem. 66:47-63.
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Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:

Ask a Scientist
⚛ Question: What is the detection limit? In particular, the number of bacteria in a 1mL sample or the quantity of ATP in a 1mL sample.

The assay measures the ATP produced by the bacteria. Most bacterial cells contain approx. 2x10-18 mol ATP per cell. Light emission is linear within a range of approx. 10 fg to 10 ng. The exact limits of the detection range will depend, of course, on the measuring device used, but theoretically this amount of ATP is similar to detection of about 1 bacterium. (10 fg = 10-14 gram and MW ATP = 507).

⚛ Question: Can we perform life / dead determination of bacteria in the sample?

Yes, you can use this assay to measure live:dead counts. You may wish to use some method to count “dead” cells independently (i.e. see our Kit M1891 for example), but the protocol describes the M1573 kit for ultrasensitive use in measuring bacterial counts after treatment with certain antibiotics, for example.

⚛ Question: Our culture media contains lipids. Will the lipids interfere with the assay?

Lipids in low concentrations should not affect the assay. Nonetheless, it will be good to run a control with and without lipid just to ensure any effect.

...Back to Top
Ask a Scientist